Biography of Robert Noyce, 1927 - 1990

Robert Noyce is credited as being the co-inventor of the integrated circuit aka the microchip along with Jack Kilby. A computer industry pioneer, Robert Noyce was the co-founder of both the Fairchild Semiconductor Corporation (1957) and Intel (1968). It was at Fairchild Semiconductor, where he was the General Manager, that Robert Noyce invented the microchip for which he received patent #2,981,877. At Intel, Robert Noyce managed and oversaw the group of inventors that invented the revolutionary microprocessor. Robert Noyces Early Life Robert Noyce was born on December 12, 1927, in Burlington, Iowa. He died on June 3, 1990, in Austin, Texas. In 1949, Noyce received his B.A. from Grinnell College in Iowa. In 1953, he received his Ph.D. in physical electronics from the Massachusetts Institute of Technology. Robert Noyce worked as a researcher for Philco Corporation until 1956, when Noyce started working for the Shockley Semiconductor Laboratory in Palo Alto, California, making transistors. In 1957, Robert Noyce co-founded the Fairchild Semiconductor Corporation. In 1968, Noyce co-founded the Intel Corporation with Gordon Moore. Honors Robert Noyce was the co-recipient of the Stuart Ballantine Medal from the Franklin Institute for his development of integrated circuits. In 1978, he was a co-recipient of the Cledo Brunetti Award for the integrated circuit. In 1978, he received the IEEE Medal of Honor. In his honor, the IEEE established the Robert N. Noyce Medal for exceptional contributions to the microelectronics industry. Other Inventions According to his IEEE biography, Robert Noyce holds 16 patents on semiconductor methods, devices, and structures, including applications of photoengraving to semiconductors, and diffused- junction isolation for ICs. He also holds the basic patent relating to metal interconnect schemes.

The Importance Of Being Earnest By Oscar Wilde - 1859 Words

Meghan DuPree English 2322 Bardenhagen Research Paper The Importance of Being Earnest In the play, The Importance of Being Earnest by Oscar Wilde, there is a lot of humor that can be found. Specifically, developed behind the characters in this play that display many instances of irony and how important it was to fit into the â€Å"status quo† of this time period. There are specific behaviors from the characters of Lady Bracknell, Gwendolen, and Algernon that portray Wilde’s opinion of society during the Victorian Age. The attitude of these characters is snobbish and their manners display double standards and superiority. The play’s use of mockery and irony of these satirical situations is meant to publicly ridicule the self- loving attitude of the upper class while exposing their true absence of intelligence which causes their absurd social behaviors The Importance of Being Earnest is about a man named Jack Worthing who works several jobs in his town servicing other people. For many years, Jack has pr etended to have a brother named Ernest who is supposedly off living a life on the edge on the pursuit of happiness, while managing to get into constant trouble. What Jack’s community doesn’t know, is that Ernest is just a made up person whom Jack uses as an excuse to leave work anytime he wants and to visit his lover Gwendolen. In the beginning, no one else knows that Ernest is actually Jack’s secret identity, until later in the play when Jack meets Algernon, who becomesShow MoreRelatedThe Importance Of Being Earnest By Oscar Wilde707 Words   |  3 PagesWebsters dictionary defines earnest as â€Å"characterized by or proceeding from an intense and serious state of mind. Which can be considered a pun since thought this play we see the characters being more apathetic. The Importance of Being Earnest is the story of Jack Worthing is the main character and the protagonist of this play. He is a well of business man who lives in the country and is very well respec ted there. But Jack has a secret he lives another in the city of London where he claims to goRead MoreThe Importance Of Being Earnest By Oscar Wilde1750 Words   |  7 PagesHidden Symbols in The Importance of Being Earnest The Importance of Being Earnest written by Oscar Wilde takes place in 1895 and exposes the hypocritical social expectations of the end of the Victorian era. During the Victorian period, marriage was about protecting your resources and keeping socially unacceptable impulses under control. The play undeniable reveals and focuses satire around differences between the behaviors of the upper class and that of the lower class. Oscar Wilde uses comedic symbolismRead MoreThe Importance Of Being Earnest By Oscar Wilde913 Words   |  4 Pagesmake them known. This concept has come to be the brick and mortar of the wry play The Importance of Being Earnest by Oscar Wilde The significance of the notion of being earnest is contradicted in the play, through Wilde’s clever use of words, characters digression of societal normalcy, and triviality of Victorian concepts. Cynical character Algernon asserts that women of Victorian society reinforce the importance of orderly money as a type of social contract. On page 3, it is quickly established theRead MoreThe Importance Of Being Earnest By Oscar Wilde975 Words   |  4 PagesThe Importance of Being Earnest is a play written by Oscar Wilde about a man named Jack who lies about his identity and ends up creating huge confusion about who he really is. The biggest notion that appears throughout the play is about character. There are many instances where the characters of the play lie about their identities and pretend to be people they are not. Oscar Wilde does this throughout the play in order to explain how one’s identity can be made up. One is not born with an identity;Read MoreThe Importance Of Being Earnest By Oscar Wilde773 Words   |  4 PagesIn the play by Oscar Wilde â€Å"The Importance of Being Earnest†, Wilde takes a comedic stance on a melodrama, portraying the duplicity of Victorian traditions and social values as the modernism of the twentieth century begins to emerge. The idea of the play revolves around its title of the characters discovering the importance of being earnest to their individual preferences. The author uses the traditional efforts of finding a marriage partner to illustrate the conflicting pressure of Victorian valuesRead MoreThe Importance Of Being Earnest By Oscar Wilde1293 Words   |  6 Pagescarrying yourself, many of which was not the must enjoyable of ways and lacked some fun that many need in their life. This forced many to split their Public life from the Private one. Written in the Victorian Era, the works of The importance of being earnest by Oscar Wilde, Dr. Jekyll and Mr. Hyde by Robert Louis Stevenson ,and Frankenstein by Mary Shelley displays how the characters need to keep be kept their Private lives separate from their Public lives in order to fit into their strict VictorianRead MoreThe Importance Of Being Earnest By Oscar Wilde1318 Words   |  6 PagesSocial Status in Persuasion and The Importance of Being Earnest Social status refers to a person s position or importance within a society. I have done some research and have acquired information over the way social status is addressed in both the writings of Jane Austen and Oscar Wilde. In the novel Persuasion we can see how the characters go beyond their means to uphold their title and social value. In the play The Importance of Being Earnest we can see how the social rank and wealth of a personRead MoreThe Importance Of Being Earnest By Oscar Wilde1364 Words   |  6 PagesIn order to fully understand the meaning of â€Å"The Importance of Being Earnest† and its importance in its time, one must look at Oscar Wilde’s background in relation to the Victorian time period. Biography.com states that Wilde had a very social life, growing up among influential Victorians and intellectuals of the time. As he grew older and became a successful writer, he began engaging in homosexual affairs which was a crime during the 19th century. He e ventually started a relationship with AlfredRead MoreThe Importance Of Being Earnest By Oscar Wilde1382 Words   |  6 Pagesappeared to be strict. The Importance of Being Earnest, by Oscar Wilde, a nineteenth century author who was one of the most acclaimed playwrights of his day, is a play set in the Victorian time period that demonstrates how trivial telling the truth was. Different characters throughout Wilde’s play establish their dishonestly through hiding who they really are and pretending to be someone whom they are not. In an essay titled â€Å"From ‘Oscar Wilde’s Game of Being Earnest,’† Tirthankar Bose describesRead MoreThe Importance Of Being Earnest By Oscar Wilde1243 Words   |  5 Pagesexuberant nonconformist and controversial playwright, eminent author Oscar Wilde produced critically acclaimed literary works that defined the essence of late Victorian England. Posthumously recognized for his only novel The Picture of Dorian Gray and satiric comedy The Importance of Being Earnest, Wilde initially acquired criticism for his immoral and unconventional style of writing. Additionally, to his dismay, strife followed Wilde in his personal life as he was notoriously tried and incarcerated

Psychological Causes of Depression Free Essays

Psychological Causes of Depression The actual causes of depression are still unknown today but there are a few theories that could help explain them. It is widely believed by psychologists and scientists that all mental disorders are brought about by a complex correlation of psychological, biological, and social factors. A serious loss, chronic illness, relationship problem, work stress, family crisis, financial setback, or any unwelcome life change can ignite a depressive disorder (Psychologyinfo. We will write a custom essay sample on Psychological Causes of Depression or any similar topic only for you Order Now om). Depression is a serious disorder in the United States and has only become more prevalent among individuals as their lives become more stressful and overwhelming. One theory for the cause of depression is the bio-psycho-social model of causation and is the most commonly recognized theory for the cause of disorders such as depression by professionals. As stated earlier, it consists of a complicated correlation of psychological, biological, and social factors. This can be caused by fluctuated levels of hormones, which would explain why many people first experience depression during puberty. (Grohol, 2006). The exact causes of depression are vast and unknown. Some types of depression have been found in families from generation to generation, which may possibly suggest that it can be inherited (Grohol, 2006). With that said, major depression seems to be present generation after generation, in some families, but not with a frequency that suggests clear biological causes. Furthermore, it also occurs in people who have no family history of depression (Psychologyinfo. com). This is also found in people with bipolar disorder. A study on family members that belong to particular families of each generation that develop bipolar disorders, has found that those with the illness have a somewhat different genetic makeup than those who do not have the disorder. Nonetheless, not everybody with the genetic makeup that is subjective to bipolar disorder will contract the disorder. Additional factors such as stresses at home, work, or school, are also involved in the disorder’s onset (depression-guide. com. , et al. ). An assortment of psychological factors appears to play a part in the susceptibility to these unrelenting types of depression. People who have low self-esteem, are not optimistic, and readily overwhelmed by stress, are prone to depression (Grohol, 2006). More than likely, these psychological factors are completely accountable for other forms of mild and moderate depression, particularly reactive depression. Reactive depression is generally diagnosed as an adjustment disorder during treatment. Social learning factors also demonstrate why psychological complications appear to occur more regularly in family members, throughout generations. For example, if a child is raised in a pessimistic household, in which discouragement is frequent and encouragement is not, the child will establish a vulnerability to depression as well. (Psychologyinfo. com. , et al). Recently, researchers have found that physical changes in the body can be paired with psychological changes as well. Medical ailments such as a cancer, Parkinson’s disease, stroke, heart attack, and hormonal disorders can bring about a depressive illness. This can result in the ill person to feel unconcerned with their health and be unwilling to further care for their physical needs. In addition, any stressful change, financial problems, relationship problems, or serious loss can activate a depressive affair (Grohol, 2006). Depression is a serious growing problem in the United States. Modern science and research is slowly helping to further decipher the disorder to help slow its growth. Over 9. 2 million Americans have major or clinical depression. At an economical standpoint, depression is a major problem and needs to be accounted for, with an estimated $30. 4 billion spent annually on depression related treatments, medication, and diagnostics. Surprisingly, the World Health Organization estimates by the year 2020, depression will be the number two cause of, â€Å"lost years of healthy living,† worldwide. As our populations keeps rising and our economy worsening, we can only hope that Psychologists help diminish the illness of depression. References â€Å"Cause of Depression: different causes of depression. † Depression Treatment, Medication, Help, Symptoms: Anxiety Attacks Depression Test, Types, ADHD Causes. Web. 14 Nov. 2009. . â€Å"Causes of depression. † Psychology Information Online. Web. 14 Nov. 2009. . Grohol, John M. â€Å"The Causes of Depression | Psych Central. † Psych Central – Trusted information in mental health and psychology. 6 Dec. 2006. Web. 14 Nov. 2009. . How to cite Psychological Causes of Depression, Essay examples

Greeks Essay Example For Students

Greeks Essay I have decided to visit a Greek Orthodox Church, Saint Constantine and Helen Greek Orthodox church. I went alone because I figured that this would not draw too much attention during the service. I tried to find a near by church that will give the service partially in English. When I found one near me it was my luck that I went at the time when it was all spoken in Greek. The English service was at six in the evening, and the all-Greek service started at ten in the morning. So to my discouragement I was at a lost at any attempts that I preplanned to adapt to this new environment. I didnt even get the name of the priest. While I was there sitting in the back I was able to dissect some of the norms of the church and the culture with in. The church was external appearance was quit different than other churches that I have seen. It was basically a big white stucco block with a gold dome on top and four decorated spikes on each corner of the building and surrounded the dome. The people of course were all Greek and were very well dressed. Most men and children all wore suits, and If not they had some kind of vest on. I dont know if this was some kind of rule or tradition, but it mostly seemed as a respect to God. The women all wore the basic dresses, and all the skirts fell down below the knee.

Affrimative Action Essays

Affrimative Action Essays Affrimative Action Essay Affrimative Action Essay Affrimative Action Affirmative Action Affirmative action is a plan made to end discrimination by guaranteeing minorities will be hired. The United States uses this practice to hire, but the qualifications of such people are occasionally overlooked. Many people believe that affirmative action is a very effective plan. The other people who oppose such action include people of various minorities, as well as many others who have been wronged by the plan. We live in a small town where there are very few minorities but in the big cities, it?s a very big deal. In several cases, this plan causes minorities to be thought of as being under qualified when hired and it also causes a new minority, the white male. Our government needs to acknowledge the fact that affirmative action is not putting an end to discrimination. This plan has succeeded in creating new minorities and more reasons for discrimination. Affirmative action frequently causes qualified employees to be looked down upon because some believe them to be affirmative action hires. My boss at work is a woman and as a result I have encountered many discriminatory comments pertaining to her position. The first remark I usually receive suggestions that she was hired for her position affirmative, action, minorities, people, plan, hired, because, qualified, very, job, discrimination, certain, believe, woman, white, upon, states, position, minority, male, made, hiring, good, gender, causes, boss, being, been, whether, united, system, standards, should, remark, received

Wuthering Heights comparitive essays

Wuthering Heights comparitive essays The theme of romance was present in the short story Skating Party and Wuthering Heights. In these stories the love the main characters experience a passion for one another that exist entirely outside social and moral conventions. In each case, the main characters act on their passions without full knowledge of the power their passions hold on them. Later on they make an important discovery about the power their passions has on them. The power of passion is progressively exposed in both Skating Party and Wuthering Heights by a series of involuntary and voluntary choices made by the main In both Skating Party and Wuthering Heights the main characters experiences a powerful attraction to another person. In Wuthering Heights, Heathcliff and Cathy experience a passion so intense that it takes over their lives and blurs their sense of reality. This type of passion is shown in the risk Heathcliff took when he visited Cathy at the Grange while she was dying. Cathy so desperately loves Heathcliff that she doesnt want him to leave, even though Edgar is coming back. When Nelly try to persuade Heathcliff to leave he refuses and stays until Edgar comes back. This is a risk since Edgar hates Heathcliff and doesnt want him in his house. In Skating Party Nathan loves Delia, but the reader isnt sure if Delia feels the same way towards him. We can see the passion that Nathan feels towards Delia when he saves her after falling through the ice instead of Eunice. We can see that Nathans passion for Delia caused the death of his fiance at the end because he reached for the hand that didnt have rabbit fur around the cuffs, which was Delias. Through these experiences, we can see that the love that main characters feel for one anoth ...

Global organization in Europe Essay Example | Topics and Well Written Essays - 2000 words

Global organization in Europe - Essay Example In the body care section the company holds the top position in the European bath and shower market, in households side they are having one of the world famous shoe polishes named Kiwi. The company is trying to settle the market of branded apparel, which includes men's and boy's wear, also sports wear. The thing that should be kept in mind at this stage is that the Sara Lee is making most of those products in which brand switching is common, and there are relatively very few differences between the competitor's products and the company's products, for example the shoe polish Kiwi is having competition with Cherry Blossom, if a consumer would go to buy Kiwi and would not find it in a shop, he would buy cherry blossom rather than going to an other shop and look for Kiwi, this brings us to Imperfect Competition, according to Lipsey (1997): This is the commonest characterization of real markets. Imperfectly competitive markets often involve products that are similar but not identical and for which there are a finite number of potential producers, each of which can influence the others by its own behavior. (p. 11) So this implies that the competitors do the first impact on trade, it means that the company has now to think about the sales quotas and forecasts, more specifically how much would they produce in the future so that there is also the minimum level of goods return and maximum levels of profit can be achieved, the next most important thing is the structure, nowadays the companies are having multiple products, so what they should know is how to maintain the product line and length as a product can damage the other products of the same firm in terms of sales and value. "Firms are trying to make the best use of available resources and this is an optimization problem familiar in economics. Issues of optimal employment, investment, and structure are all susceptible to economic analysis"(Lipsey, 1997). After wards there are more things to worry about, like the growth rate of GDP should be kept in mind as if any company that is low in demand or even having an exceeding demand can put some im pact on GDP, Like in case of Sara lee the food production depends on the past sales records, else at times the senior executives take decision when they have to enter in some new market, specially it creates a lot of fuss when new product is to be launched in some area, it has a lot to do with investment not only in production but also in conducting research so that the chances of product failure can me minimized. The biggest advantage of having such operating organization in UK is that the local goods are promoted, it means that more the local goods are used the less the country relies on import, reminding that the company is still local, and the goods are not imported in the case of Sara Lee, the production of goods is done by the firms that are being operated with in UK by taking the license from the main Sara Lee Corp. Another good advantage of this organization is that the competition between Sara Lee and other firms is maintained, reminding that competition is something in whi ch the customers get the benefit as an outcome, a good example

Enviromental issue Research Paper Example | Topics and Well Written Essays - 2000 words

Enviromental issue - Research Paper Example The severity of air pollution in China and the extent it creates serious health hazards are evident from alarm that its people are warned are warned to stay indoor in order save their breath from contaminated air. This piece of paper considers three articles that talk about air pollution and its effects on human environment and sums up major emphasizes from those articles. This paper is to analyze how media, based on these articles, played its role in spreading about this severe environmental issue and what solutions have been recommended by them to make people aware of its severity. Air Pollution: a superpower in to a ‘worst-land’? (defining the issue) BBC in August 2006 reported, in an article titled ‘China hit by raising air pollution’ that China has become world’s largest sulphur dioxide polluter, due to that it has emitted 26 million tons of the gas in 2005, showing a 27% increase from the figure of 2000. This excesses emissions of sulfur dioxide has been causing acid rains in different part of China and other parts of the world. As Kahn and Yardley noted in their article- ‘As China Roars, Pollution Reaches Deadly Extremes’, published in New York Times (2007), China’s air pollution itself is a cancer leading to a large number of deaths. Costs yield benefits. Its economically true and naturally very evident, especially from China’s case. This fact has been highlighted in their article. China, being unparalleled in the history, emerged as a major industrial power, but created a legacy of environmental issue, air pollution that may negate all the credits its economic growth has conferred. The article has defined and described the issue in detail. Pubic health is extremely affected by air pollution as it alone causes hundreds of thousands deaths every year. One of the most critical and perhaps more dangerous effects of this pollution is that around 500 million people lack access to safe drinking water . Both cities and rural areas have been turned to be toxic areas where only around one percent of air is considered quite safe. In industrial cities, people more often are not able to see sun, not due to raining or sky being cloudy, but due to the pollution that made the air and sky dry. The atmosphere has largely been contaminated. On one side, the economy gains ever-recorded growth and large multinationals still attempting to grab the slice of this international marketing pie, whereas on the other side, people die for bad breath due to air pollution. As this article emphasized, China’s pollution is not only China’s problem. It is adverse effects have widened to other countries. Dangerous chemical elements like sulfur dioxide and nitrogen oxides that are spewed from China’s coal plants caused acid rains in Seoul, South Korea, Tokyo etc. Tatchell in 2008 wrote in their article titled ‘The Pollution Marathon’, published in the Guardian, about the iss ue of China’s air pollution and its drastic impacts on every one’s life in the Guardian newspaper. This article starts with Ethiopian long-distance runner Haile Gebrselassie’s decision not to participate in 2008 Olympics held in China. He was highly concerned about the extremely hazardous air pollution in China and its impacts on his health if he stays few days

Write a management report Assignment Example | Topics and Well Written Essays - 1250 words

Write a management report - Assignment Example a key factor to the growth of the resort and thus it was recommended of creating an environmental friendly ecosystem which was further enhanced by usage of wind to generate more energy to pump to the power grid. Jiminy peak, a snowboarding and skiing resort found in the Berkshire Mountains of the western parts of Massachusetts is the largest resort of its kind in the states of southern New England. Founded in 1948, its offers skiing and snowboarding, outdoor activities such as Mountain bike riding and many more sporting activities. It gained four season resort status in the year 2005 symbolizing its growth. It is strategically placed between some of the biggest cities around, for example, it is just three hours away from Boston and New York City and about an hour’s drive from other major cities such as Springfield, Massachusetts and Albany. Jiminy Peak has grown to become one of the most important ski destinations due to some key factors that play to its favor such as its strategic location between such vast cities, the good managerial decisions being undertaken within this organization leading to its much deserved development. Another factor that makes Jiminy Peak to be a key attraction is the fact that it is very vast, purely because of the developments that have taken place leading to so many activities being incorporated in these 170 acres. The number of snowboarding and ski trails totals to forty five, has three terrain parks six person chairlifts of high speed and nine lifts. Much of the success of this resort is attributed to the marketing teams, who have been able to market the resort and make it accommodate high numbers of sport activity lovers even in the summer. For this purpose, the resort has set up a mountain coaster, the first resort to do so in the whole of the East Coast, they have also managed to put up more summer sport devices such as alpine super slides, gigantic swings and more activities such as bungee jumping, rock climbing, scenic

Analysis of Peripherin-2 Cone Mutation V268I

Analysis of Peripherin-2 Cone Mutation V268I Introduction: 1.1 Composition of Retina: Eye an organ which is known for its sensory function Vision, serves as a basis for perception of the world outside. Three layers of tissues enclose the eye, outermost layer sclera, middle layer choroid and inner layer retina. Central macular region of the retina called as fovea consist of photoreceptor cells, where maximum visual acuity is possible due to minimal light scattering. Iris found in region between the cornea and lens acts like an aperture controlling the amount of light that could enter and pass through retina (Siegel and Sapru.2011). Retina consists of following layers: The outer most primary epithelium layer with pigmented melanin cells absorbs uncaptured light preventing reflection back to rest of retina. The later parts of retina layers involved in sensing and processing of light stimulus are the layers with photoreceptor cells- light sensitive region with rod and cone cells, external limiting membrane, outer nuclear layer, outer plexiform layer, inner nuclear layer, inner plexiform layer and inner most layer with ganglion cell axon fibers. Optically as light when imaged onto retinal photoreceptor layer, light passing through this layer with rods and cones converts light stimuli to electric signal through horizontal cells, further processed through bipolar, amacrine before they reach ganglion cells. From the axons of Central Nervous System (CNS) ganglion cells information is passed in the form of action potential to optic disc and optic nerve signal is received by brain’s visual center. (Ayoub, 2008). 1.2 Composition of Photoreceptors: Retina is made up of two different types of photo receptors- Rods and cones, the ratio of these photoreceptors vary in different species depending on its habitat being diurnal or nocturnal, in human’s rod cells are in majority with cone cells in a ratio of 20:1 (Mustafi et al., 2009). Rods and cones consist of outer segment, inner segment and synaptic terminal. Towards outer surface retina outer segments are located, these segments contain membranous discs arranged in the form of a stack, and these regions are involved in photo transduction by detecting photons and converting them into electrical signals. Inner segment consist of nucleus, both segments are integrated by a stalk containing microtubules. The synaptic terminal carries out synaptic contact with other cells (Siegel and Sapru. 2011). Retinal pigment epithelial cells phagocytize the aged disks which are shed from distal end when new a new disk is added to the base (Young. 1967). Fig: 1 Structure of rod and cone cell. In rods outer segment disc membrane is separated by plasma membrane, disks consist of enclosing lipid bilayer compartment called as disk lumen or intra distal space. Lamellar region of the disk is joined by curved rim region, lamellar region consist of photoreceptor protein, Rhodopsin – an integral membrane protein, and curved rim regions consist of filaments extending to cytoplasmic regions of adjoining disks maintaining structure of outer segment. Rim regions also contain membrane proteins Rim-1, a high molecular weight protein and two other lower molecular weight proteins Peripherin/rds and Rom-1. In cones discs are not completely formed, they are adjoined and are continuous with plasma membrane. (Molday. 1994). These two proteins Peripherin/rds and Rom-1 are homologous in structure, peripherin/rds is expressed both in rods and cones while Rom-1 is only found in rods, they can interact to form dimers and tetramers. (Elizabeth. 2005) Cones are known to be responsible for vision during daylight and mediate color vision, while rods do not. Axial structure of cones makes them capture light efficiently during day, with fast responsive membrane potential with less integration time. There are three types of cones L, M, S cones which are specific to a region of visible spectrum to which they are found to be sensitive. L cones are sensitive to ÊÅ ½max~ 553-565, M cones are sensitive to ÊÅ ½max~ 530-537, S cones are sensitive to ÊÅ ½max~ 415-430, frequency of photons. (Mustafi et al., 2009). Rods are specialized to work under dim light conditions, rhodopsin is the most abundant protein found in the rods constituting about 85 % of total protein in rod outer segments. (Elizabeth. 2005). Circuit transmitting information to ganglion cells is different in rods and cones, each rod bipolar cell is connected to many rod cells, and many rod bipolar cells are connected by amacrine cell. More convergence make rods better detector of light but reduces spatial resolution. In cones it’s less convergent as each ganglion cell receives input from only one cone bipolar cell, which is connected to single cone cell (Mustafi et al., 2009). Signal transduction in Photoreceptors: The signaling cascade in photoreceptors involves from light absorption till signal generation by membrane polarization, resulting in controlling the rate of release of neurotransmitter through synaptic terminal. In Dark light condition gyanylate cyclases (GCs) actively produce high Cyclic guanosine monophosphate (cGMP) levels, There by the cGMP sensitive cyclic nucleotide-gated (CNG) channel is in its open state and gives rise to the influx of Na+ and Ca+2. Rods and cones are depolarized to ~35 to 45 mV, where outer segment membrane channels are open permeable to Na+ by effluxing k+ balancing cations. This constitutes a circuit known as â€Å"Dark or circulating current†. In this condition with the channel opened for influx of ca+2 initiates the release of neurotransmitter from synaptic terminal. In light conditions cation channel in outer membrane is closed, thereby membrane hyperpolarizes towards equilibrium potential for k+, causing halt in neurotransmitter release (Jindrova. 1998). When photon is absorbed by Rhodopsin- a 40 kDa protein belonging to G protein coupled receptors, consist of a protein opsin, a single polypeptide with seven transmembrane helical segments forming three cytoplasmic loops and a chromophore 11-cis retinal, which binds to lysine, 296 residue on opsin’s seventh helix. In rods and cones photon absorption leads to isomerization of 11-cis retinal form to all trans retinal confirmation and in the surrounding opsin repositioning of cytoplasmic loops occur which in turn activates the protein to Metarhodopsin II (R*) state, which stimulates the heterotrimeric G protein transducin (Hargrave et al.1993; Scot and John . 2008). Preceding from here all the steps occur in dark state, Transducin stimulates cGMP phosphodiesterase (PDE6) to hydrolyze cGMP to 5’GMP by dissociation of ÃŽ ³ subunit from PDE ÃŽ ±ÃŽ ² subunits, resulting a vast increase of 5’GMP and decrease in cGMP concentration leading to the closing of the CNG channel. As a consequence, the outer membrane hyperpolarises to ~-70 mV and release of neurotransmitter is halted to bipolar cells, finally light signal information is sent by termination of transmitter release to the brain. For regeneration of dark current, the Rod cell needs to restore back the concentration of cGMP which is regulated by concentration of Ca+2 and by gyanylate cyclases (GC), Ca+2 molecules are bound by gyanylate activating proteins. In light conditions, where the CNG channels are closed and Ca+2 levels are low, the low level of calcium activates gyanylate activating proteins, which in turn stimulates gyanylate cyclases leading to production of cGMP. Increased level of cGMP causes to open CNG channel and dark current is restored (Jindrova. 1998). Additional mechanisms are found to be involved to restore the molecules from active to inactive state. Inactivation of Rh* by phosphorylation, catalyzed by rhodopsin kinase (Chenet al.1999). Phosphorylated rhodopsin is blocked by the protein arrestin by binding to it preventing activation of transducin, resulting in breakdown of activated rhodopsin (Xu et al,. 1997). The all trans retinal disassociates from opsin, diffuses to cytosol transported to outersegment then into pigmental epithelium where it is reconverted to 11- cis retinal, then the recycled 11 -cis retinal is transported back to outer segments. Peripherin- 2: This membrane protein is named on basis of localization studies done in 1987, which revealed that this protein was found localized around the periphery region of the outer segments, disks in rods and lamellae or disks in cones. (Molday et al,. 1987). In humans peripherin/rds is encoded by cDNA Peripherin/rds with open reading frame of 346 amino acids, weighing 39.3 KDa (Travis et al., 1989). Peripherin is an integral membrane glycoprotein having four trans membrane domains. When photoreceptor cells fail to develop in outer segments it results in retinal disorder named retinal degeneration slow (rds). Amino acid sequence of peripherin in bovine photoreceptor cell is 92.5% identical to protein encoded by rds gene in wild type mouse. Localization of these proteins was also found to be in rod outer segment (ROS) membranes, implying the role of peripherin-2 as a cause in retinal disorder. (Connell et al, .1991). Peripherin protein is also known and addressed with many other names like pho toreceptor peripherin, peripherin-2, peripherin/rds, rds/peripherin and rds. The two dimensional structure proposed consisted of four membrane spanning domains, with its N and C terminals located intracellular in extradiscal space, two extra cellular loops D1 and D2 ( EC1 and EC2) are localized with each containing a N-linked glycan in intradiscal space. The D2 loop among four species mouse, rat, human and bovine is found to be 92% identical, predicted that evolutionary drift in this loop could be a reason for its involvement in protein -protein interactions. (Connell and Molday, 1990; Travis et al., 1991). Fig: 2 Structure of Peripherin-2. D2 loop in intradiskal region is found to be key in protein folding and tetrameric subunit formation, changes in amino acids in this loop resulted in either protein misfolding or reduction in sediment coefficient of protein or even both, seven cysteine residues found in this loop to be important for intramolecular disulphide bonds, one of the cysteine residue is responsible for polymerization of tetramers (Loewen and Molday, 2000). When seven conserved cysteine residues were replaced they showed abnormalities, C214S linked mutant for adRP was not normally folding and interacting with rom-1 protein to form tetramer. While in C150S mutant failed to form intra molecular disulphide bonds, these results revealed that cysteine residues were crucial for folding of protein and subunit assembly, failure of these functions are linked to retinal disorders like adRP. (Goldberg et al., 1997). Human rom-1 protein with 351 amino acids exhibiting 35% identity to peripherin-2, hydropathy profiles revealed that rom-1 and peripherin-2 have a similar topology with four transmembrane domains, one of differences noted in the both the proteins were consensus sequence for N-linked glycosylation is absent in the rom-1 (Molday. 1994). Peripherin homologous protein rom-1 forms disulfide-linked dimers with each other (Bascom et al.,1992). Peripherin-2/rom-1 core native complex was found to exist as a tetramer, membrane curvature was found to induce with tetramer formation when they were reconstructed in lipid vesicles (Kevany et al,. 2013). Peripherin-2 associates with itself and also with its homologue rom-1 in forming homo and heterotetramers core, these tetramers when linked intramolecularly together by disulphide bonding forms octamers and higher order oligomers (Loewen and Molday. 2000). A highly conserved region within the C-terminal domain of peripherin/rds was found to be import ant for membrane fusion, it forms a complex with melanoregulin (MREG) onto the last five residues of the C-terminus (Gln341-Gly346), membrane fusion is important in organelle biogenesis, disc morphogenesis and disc shedding. (Boesze-Battaglia et al., 2007). Peripherin/rds plays a major role in rod and cone outer segment morphogenesis. In mice, absence of peripherin/rds leads to develop normal photoreceptor inner segments but they fail to form outer segments, these outer segments undergo apoptosis (Nir and Papermaster. 1986). Peripherin- 2 is found to have differential roles in rod and cone cells with respect to their binding partners. Peripherin 2 found to be binding with the Glutamic acid rich protein (GARP) portion of ÃŽ ² subunit of rod CNG channel. This interaction is predicted to anchorage disc rim and rod plasma membrane, but in cone CNG channel such interaction was not observed, as GARP is not expressed in cone CNG channel or in other from. (Conley et al,.2010). Peripherin-2 links CNGB1a CNG channel subunit to rhodopsin, these three forms a complex localizing its contact between disk rims and plasma membrane regions. FRET experiments revealed that transmembrane -4 (TM4) of periperin-2 is key for rhodopsin interaction. In peripherin-2, G266D mutation specific to TM4 region, eliminated the specific binding capacity of peripherin-2 with rhodopsin (Becirovic et al,. 2014). Peripherin-2 being a multifunctional protein and mutations in peripherin/RDS gene results in a broad spectrum of retinal disorders like macular dystrophies, cone and cone-rod dystrophies and retinitis pigmentosa. Studying Peripherin/RDS mutations and protein structure elucidates pathophysiological mechanisms underlying these retinal disorders for effective therapeutic intervention (C.J.F. Boon et al,. 2008). Zscan29: At present, very less information is known and available regarding the function, structure and localization of this protein Zscan29. mRNA specific for this protein were found to be expressed in retina through RT- PCR experiments from mice different tissues. In mice 5 different isoforms of this protein were found, with longest isoform having 869 amino acids (aa), and other isoforms were of 834 aa, 548 aa,265 aa,206 aa. The isoform with 265 aa was found to interact with the C- terminus of CNGB1a sub unit of CNG channel. In long isoforms with 869aa and 834aa, a SCAN domain, a DUF2 domain, 2 GT-1 domains and 6 zinc finger domains were found. The scan domain is predicted to be a DNA binding domain and involved in transcription regulation, as this domain in other proteins were also found to be associated as transcription factors. DUF-2 Domain function is unclear and yet to be known, Two GT-1 domains of 79 aa each, were predicted to play a major role in light sensing mechanisms by binding GT trihelix transcription factors. (___citation__). In Arabidopsis GT-1 is predicted to respond to light signals via calcium dependent phosphorylaton, after GT-1 binding to GT cis element of light inducing gene (Bauer et al). Fig: 3 Isoforms of Zscan29 protein with its domains. Aims of this work: Analysis of Peripherin-2 cone mutation V268I: Verification of the interaction of WT peripherin-2 and peripherin-2 with mutation at V268I position with S-opsin, M-opsin and Rhodopsin via co immunoprecipitation from HEK293 cells. Interaction and localization of Peripherin-2, V268I mutation in mouse retina. Analysis of Peripherin-2 mutations at position P210: Verification of the interaction of peripherin-2 protein with mutations at P210 position with wild type Peripherin-2 and its homolog Rom-1 via coimmunoprecipitation from HEK293 cells. In vitro imaging of HEK293 cells expressing peripherin-2 protein with mutation at P210 position. Expression and localization of Peripherin-2, P210L and P210R mutations in mouse retina. Affinity purification of Anti-Zscan29 antibody and checking antibody efficiency.

The Cosmetic Industry: Selling Illusion :: Argumentative Persuasive Argument

The Cosmetic Industry: Selling Illusion The makeup industry is booming these days. With the ability to make skin appear smoother, eyelashes look longer, and make lips a different color, makeup continues to create a distorted reality for people around the world. The cosmetic industry is still going strong because our culture continues to promote its use. For example, women are expected to wear makeup for certain occasions. They are expected to wear makeup at work, when they go out to eat, and nearly every other situation that involves an appearance in public. If a woman is seen not wearing makeup in public, then she is considered a deviant, and others look down on her. The entire makeup market, the value of eye, lip, nail and face makeup, was $23.7 billion in 1998. This market has also experienced a compounded annual growth rate (CAGR) of 3.7 percent from 1994 to 1998. (Bucalo) One obvious explanation for this large value of the industry is that makeup is extremely appealing to consumers. It is a great way to cover up the imperfections in one's appearance. The "need" to cover the imperfections in our face and skin is magnified by the importance that is placed on looking beautiful. It is sad, but many times people are judged by their appearance. People realize this and strive to improve themselves with makeup. They feel it is necessary to put on a mask when presenting themselves to the public because they fear that they may be inaccurately judged if they do not. Makeup has other uses, too. There are some circumstances that call for the distortion of reality, such as movies. An actor can represent a character much better with a little distortion of his true appearance. Sometimes the story line takes place over an extended period of time. An actor may need to change their appearance from young to old throughout the course of the movie. Makeup can also make it easier for a movie to seem more realistic. Can you imagine how lame Star Wars or Dracula would have been if the actors had played the parts without change from their natural appearance? Clowns also seem to have a convincing argument that makeup is useful in their line of work. In fact, the makeup that a clown wears is characteristic of their job description. A clown without makeup is like a fish out of water. Makeup is so useful for clowns because an unchanging mood can be put on their face.